ClinVar Miner

Submissions for variant NM_004972.4(JAK2):c.1849G>T (p.Val617Phe)

gnomAD frequency: 0.00037  dbSNP: rs77375493
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Total submissions: 30
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Submitter RCV SCV Clinical significance Condition Last evaluated Review status Method Comment
Mayo Clinic Laboratories, Mayo Clinic RCV001092995 SCV007134199 pathogenic not provided 2024-10-29 criteria provided, single submitter clinical testing
Institute of Human Genetics, University of Leipzig Medical Center RCV000015771 SCV007113990 pathogenic Acute myeloid leukemia 2025-11-11 criteria provided, single submitter clinical testing Criteria applied: PS4,PS3_MOD,PM1,PP4_MOD
Genetic Services Laboratory, University of Chicago RCV001092995 SCV006325264 likely pathogenic not provided 2023-12-06 criteria provided, single submitter clinical testing DNA sequence analysis of the JAK2 gene demonstrated a sequence change, c.1849G>T, in exon 14 that results in an amino acid change, p.Val617Phe. The p.Val617Phe change affects a highly conserved amino acid residue located in a domain of the JAK2 protein that is known to be functional. The p.Val617Phe substitution appears to be deleterious/possibly damaging using several in-silico pathogenicity prediction tools (SIFT, PolyPhen2, Align GVGD, REVEL). This sequence change has been described in the literature in other individuals with JAK2-related disorders in both the germline and somatic state (PMID: 26556299, 31721094, 23535062). In addition, a different pathogenic sequence change affecting the same amino acid residue (p.Val167Ile) has been described in several individuals with JAK2-related hereditary thrombocytosis (PMID: 22397670, 23535062). This sequence change has been described in the gnomAD database with a frequency of 0.075% in the Ashkenazi Jewish subpopulation (dbSNP rs77375493). Collectively, this evidence indicates that this sequence change is likely pathogenic; however, functional studies have not been performed to prove this conclusively.
Laboratory of Genetics, Children's Clinical University Hospital Latvia RCV001092995 SCV006106468 pathogenic not provided 2025-02-16 criteria provided, single submitter clinical testing
3billion RCV000015769 SCV005905726 likely pathogenic Acquired polycythemia vera 2024-02-15 criteria provided, single submitter clinical testing The variant is observed at an allele frequency greater than expected for the associated disorder in the gnomAD v2.1.1 dataset and therefore considered benign. Predicted Consequence/Location: Missense changes are a common disease-causing mechanism. In silico tool predictions suggest damaging effect of the variant on gene or gene product [REVEL: 0.88 (>=0.6, sensitivity 0.68 and specificity 0.92)]. Same nucleotide change resulting in same amino acid change has been previously reported as pathogenic/likely pathogenic with strong evidence (ClinVar ID: VCV000014662). A different missense change at the same codon (p.Val617Ile) has been reported to be associated with JAK2 related disorder (ClinVar ID: VCV000029763 /PMID: 22397670). Therefore, this variant is classified as Likely pathogenic according to the recommendation of ACMG/AMP guideline.
Juno Genomics, Hangzhou Juno Genomics, Inc RCV000763621 SCV005415821 pathogenic Primary familial polycythemia due to EPO receptor mutation; Acquired polycythemia vera; Budd-Chiari syndrome; Primary myelofibrosis; Acute myeloid leukemia; Thrombocythemia 3 criteria provided, single submitter clinical testing Absent from controls (or at extremely low frequency if recessive) in Genome Aggregation Database, Exome Sequencing Project, 1000 Genomes Project, or Exome Aggregation Consortium.;Well-established in vitro or in vivo functional studies supportive of a damaging effect on the gene or gene product.;The prevalence of the variant in affected individuals is significantly increased compared to the prevalence in controls.;Assumed de novo, but without confirmation of paternity and maternity.
Centre for Clinical Genetics and Genomic Diagnostics, Zealand University Hospital RCV001092995 SCV005328481 pathogenic not provided 2024-02-26 criteria provided, single submitter clinical testing
Genomic Medicine Center of Excellence, King Faisal Specialist Hospital and Research Centre RCV000015769 SCV004806756 likely pathogenic Acquired polycythemia vera 2024-09-02 criteria provided, single submitter clinical testing
Baylor Genetics RCV000015770 SCV003835086 pathogenic Primary myelofibrosis 2021-08-30 criteria provided, single submitter clinical testing
Revvity Omics, Revvity RCV000022627 SCV003831788 likely pathogenic Thrombocythemia 3 2022-10-03 criteria provided, single submitter clinical testing
Institute of Human Genetics, University of Leipzig Medical Center RCV000015770 SCV002765070 pathogenic Primary myelofibrosis 2022-11-24 criteria provided, single submitter clinical testing _x000D_ Criteria applied: PS3, PS4, PM1, PM5, PP4
Provincial Medical Genetics Program of British Columbia, University of British Columbia RCV000015769 SCV002320869 pathogenic Acquired polycythemia vera 2022-01-01 criteria provided, single submitter clinical testing
Labcorp Genetics (formerly Invitae), Labcorp RCV001092995 SCV002307245 likely pathogenic not provided 2026-01-22 criteria provided, single submitter clinical testing This sequence change replaces valine, which is neutral and non-polar, with phenylalanine, which is neutral and non-polar, at codon 617 of the JAK2 protein (p.Val617Phe). The frequency data for this variant in the population databases is considered unreliable, as metrics indicate poor data quality at this position in the gnomAD database. This variant is a well-known somatic change that has been reported as a recurrent variant in many individuals affected with myeloproliferative disorders (PMID: 15920007, 15781101, 15858187, 15793561, 16603627). ClinVar contains an entry for this variant (Variation ID: 14662). Invitae Evidence Modeling of protein sequence and biophysical properties (such as structural, functional, and spatial information, amino acid conservation, physicochemical variation, residue mobility, and thermodynamic stability) indicates that this missense variant is not expected to disrupt JAK2 protein function with a negative predictive value of 80%. Experimental studies have shown that this missense change affects JAK2 function (PMID: 15793561, 23535062). A different missense substitution at this codon (p.Val617Ile) has been reported to segregate with autosomal dominant hereditary thrombocytosis as a germline change in a single family (PMID: 22397670). Functional studies show that this variant results in constitutive kinase activation and cytokine hyper-responsiveness (PMID: 23535062, 22397670). In summary, the currently available evidence indicates that the variant is pathogenic, but additional data are needed to prove that conclusively. Therefore, this variant has been classified as Likely Pathogenic.
GeneDx RCV001092995 SCV001825403 pathogenic not provided 2025-10-27 criteria provided, single submitter clinical testing In silico analysis supports that this missense variant has a deleterious effect on protein structure/function; This variant is associated with the following publications: (PMID: 23425079, 25698270, 21120162, 21689158, 27777768, 28205126, 24381227, 16755940, 16293597, 29349042, 31721094, 28596259, 29641446, 23300178, 16156870, 16871278, 20182460, 24068492, 15781101, 19287384, 25157968, 24986690, 16081687, 16709929, 18394554, 24404189, 20339092, 22571758, 20631743, 23115274, 22818858, 22041374, 22829971, 22422826, 21160067, 19549988, 16341032, 26228487, 23537216, 23248577, 23057517, 19195039, 17596137, 17440677, 17194663, 16990759, 16904848, 16954506, 16926301, 15920007, 15858187, 15860661, 26556299, 19293426, 16762626, 15793561, 30944118, 33144682, 32581362, 31447099, 19327411, 27389715, 35861108, 37885353, 35150601)
CeGaT Center for Human Genetics Tuebingen RCV001092995 SCV001249759 pathogenic not provided 2026-02-01 criteria provided, single submitter clinical testing JAK2: PS4, PM1, PP4:Moderate, PS3:Moderate
Fulgent Genetics, Fulgent Genetics RCV000763621 SCV000894475 pathogenic Primary familial polycythemia due to EPO receptor mutation; Acquired polycythemia vera; Budd-Chiari syndrome; Primary myelofibrosis; Acute myeloid leukemia; Thrombocythemia 3 2018-10-31 criteria provided, single submitter clinical testing
Human Genome Sequencing Center Clinical Lab, Baylor College of Medicine RCV000015769 SCV000839961 pathogenic Acquired polycythemia vera 2018-02-08 criteria provided, single submitter clinical testing The c.1849G>T (p.V617F) variant in the JAK2 gene is commonly reported as a somatic change in myeloproliferative neoplasms (MPNs) including polycythemia vera, essential thrombocythemia, primary myelofibrosis [PMID: 15781101, 15858187, 15837627, 19074595]. Recently, this variant has also been described as one of the most common drivers of age-related clonal hematopoiesis [PMID: 27563148]. The c.1849G>T (p.V617F) variant in the JAK2 gene is classified as a pathogenic somatic variant.
PreventionGenetics, part of Exact Sciences RCV004751211 SCV005351640 pathogenic JAK2-related disorder 2024-04-30 no assertion criteria provided clinical testing The JAK2 c.1849G>T variant is predicted to result in the amino acid substitution p.Val617Phe. This variant has been reported to be present in almost all patients with polycythemia vera and more than half of those with essential thrombocytosis and primary myelofibrosis (Vassiliou 2016. PubMed ID: 27563148). Somatic c.1849G>T variants have been reported in myeloproliferative neoplasms (Ortmann et al. 2015. PubMed ID: 25671252; Rumi et al. 2014. PubMed ID: 24986690). This variant is reported in 0.058% of alleles in individuals of Ashkenazi Jewish descent in gnomAD. This variant is interpreted as pathogenic.
Molecular Diagnostics Laboratory, University of Rochester Medical Center RCV000015769 SCV004175181 pathogenic Acquired polycythemia vera no assertion criteria provided provider interpretation
Joint Genome Diagnostic Labs from Nijmegen and Maastricht, Radboudumc and MUMC+ RCV001092995 SCV002037447 pathogenic not provided no assertion criteria provided clinical testing
Laboratory of Diagnostic Genome Analysis, Leiden University Medical Center (LUMC) RCV001092995 SCV002036319 pathogenic not provided no assertion criteria provided clinical testing
NIHR Bioresource Rare Diseases, University of Cambridge RCV000427081 SCV001162251 pathogenic Myeloproliferative disorder no assertion criteria provided research
NIHR Bioresource Rare Diseases, University of Cambridge RCV001003804 SCV001162250 pathogenic Polycythemia no assertion criteria provided research
NIHR Bioresource Rare Diseases, University of Cambridge RCV001003803 SCV001162249 likely pathogenic Primary myelofibrosis; Splenomegaly no assertion criteria provided research
OMIM RCV000022628 SCV000043917 affects Primary familial polycythemia due to EPO receptor mutation 2014-08-07 no assertion criteria provided literature only
OMIM RCV000022627 SCV000043916 pathogenic Thrombocythemia 3 2014-08-07 no assertion criteria provided literature only
OMIM RCV000015772 SCV000036038 risk factor Budd-Chiari syndrome, susceptibility to, somatic 2014-08-07 no assertion criteria provided literature only
OMIM RCV000015771 SCV000036037 pathogenic Acute myeloid leukemia 2014-08-07 no assertion criteria provided literature only
OMIM RCV000015770 SCV000036036 pathogenic Primary myelofibrosis 2014-08-07 no assertion criteria provided literature only
OMIM RCV000015769 SCV000036034 pathogenic Acquired polycythemia vera 2014-08-07 no assertion criteria provided literature only

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