ClinVar Miner

Submissions for variant NM_002709.3(PPP1CB):c.146C>G (p.Pro49Arg)

dbSNP: rs886037952
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Total submissions: 29
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Submitter RCV SCV Clinical significance Condition Last evaluated Review status Method Comment
ClinGen RASopathy Variant Curation Expert Panel RCV004732471 SCV005367861 pathogenic RASopathy 2024-09-17 reviewed by expert panel curation The c.146C>G (Pro49Arg) variant in PPP1CB is a missense variant predicted to cause substitution of proline by arginine at amino acid 49. This variant is absent from gnomAD v4 (PM2_Supporting). PPP1CB, in which the variant was identified, is defined by the ClinGen RASopathy VCEP as a gene that has a low rate of benign missense variation and where pathogenic missense variants are a common mechanism of disease (PP2). The missense Z-score is 4.33 which is above the threshold set by the Rasopathy VCEP. The computational predictor REVEL gives a score of 0.438, which is neither above nor below the thresholds predicting a damaging or benign impact on PPP1CB function. This variant has been reported in 13 probands with features of RASopathy (PS4; PMIDs: 27264673, 27681385, 27868344, 28211982, 30368668, 32476286). This variant has been identified as a de novo occurrence with confirmed parental relationships in 9 individuals and as a de novo occurrence with unconfirmed parental relationships in 2 individuals with features of RASopathy (PS2_VeryStrong; PMIDs: 27264673, 27681385, 27868344). In summary, this variant meets the criteria to be classified as pathogenic for autosomal dominant RASopathy based on the ACMG/AMP criteria applied, as specified by the ClinGen RASopathy VCEP: PS2_VS, PS4, PM2_P, PP2. (RASopathy VCEP specifications version 1.1; 9/17/2024)
Center of Human Genetics, Hôpital Erasme RCV000490622 SCV006076751 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2025-01-01 criteria provided, single submitter clinical testing
Ambry Genetics RCV005260026 SCV005922537 pathogenic Cardiovascular phenotype 2025-03-04 criteria provided, single submitter clinical testing The p.P49R pathogenic mutation (also known as c.146C>G), located in coding exon 2 of the PPP1CB gene, results from a C to G substitution at nucleotide position 146. The proline at codon 49 is replaced by arginine, an amino acid with dissimilar properties. This variant was reported in individual(s) with features consistent with PPP1CB-related Noonan syndrome-like disorder with loose anagen hair; in at least one individual, it was determined to be de novo (Ma L et al. Hum Genet, 2016 Dec;135:1399-1409; Gripp KW et al. Am J Med Genet A, 2016 Sep;170:2237-47; Zambrano RM et al. Am J Med Genet A, 2017 Feb;173:565-567; Bertola D et al. Am J Med Genet A, 2017 Mar;173:824-828; Turner TN et al. Am J Hum Genet, 2019 Dec;105:1274-1285; Giugliano T et al. Genes (Basel), 2019 Jul;10). This missense alteration is located in a region that has a low rate of benign missense variation (Lek M et al. Nature. 2016 Aug 18;536(7616):285-91; DECIPHER: Database of Chromosomal Imbalance and Phenotype in Humans using Ensembl Resources. Firth H.V. et al. 2009. Am.J.Hum.Genet. 84, 524-533 (DOI: dx.doi.org/10/1016/j.ajhg.2009.03.010)). This amino acid position is highly conserved in available vertebrate species. In addition, the in silico prediction for this alteration is inconclusive. This variant is considered to be rare based on population cohorts in the Genome Aggregation Database (gnomAD). Based on the supporting evidence, this variant is interpreted as a disease-causing mutation.
Molecular Genetics Laboratory, BC Children's and BC Women's Hospitals RCV000490622 SCV005367958 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2024-07-09 criteria provided, single submitter clinical testing
Rady Children's Institute for Genomic Medicine, Rady Children's Hospital San Diego RCV000490622 SCV004046312 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 criteria provided, single submitter clinical testing This variant has been previously reported as a de novo heterozygous variant in multiple unrelated individuals with Noonan-like syndrome with loose anagen hair (PMID: 27264673, 27681385, 27868344, 28211982, 31474318). It is absent from the gnomAD population database and thus is presumed to be rare. The c.146C>G (p.Pro49Arg) variant is predicted by multiple in silico tools to have a deleterious effect on protein function. Based on the available evidence, the c.146C>G (p.Pro49Arg) variant is classified as Pathogenic.
Department of Endocrinology and Genetics, Fuzhou Children’s Hospital of Fujian Medical University RCV000490622 SCV002574761 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2022-09-08 criteria provided, single submitter clinical testing
Centre de Biologie Pathologie Génétique, Centre Hospitalier Universitaire de Lille RCV002274002 SCV002559115 pathogenic Neurodevelopmental delay criteria provided, single submitter clinical testing
Victorian Clinical Genetics Services, Murdoch Childrens Research Institute RCV000490622 SCV002557913 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2023-07-17 criteria provided, single submitter clinical testing Based on the classification scheme VCGS_Germline_v1.3.4, this variant is classified as Pathogenic. Following criteria are met: 0101 - Gain of function is a known mechanism of disease in this gene and is associated with Noonan syndrome-like disorder with loose anagen hair 2 (MIM#617506) (PMID: 30348783). (I) 0107 - This gene is associated with autosomal dominant disease. (I) 0200 - Variant is predicted to result in a missense amino acid change from proline to arginine. (I) 0251 - This variant is heterozygous. (I) 0301 - Variant is absent from gnomAD (both v2 and v3). (SP) 0501 - Missense variant consistently predicted to be damaging by multiple in silico tools or highly conserved with a major amino acid change. (SP) 0603 - Missense variant in a region that is highly intolerant to missense variation (high constraint region in DECIPHER). (SP) 0801 - This variant has strong previous evidence of pathogenicity in unrelated individuals. This is a recurrent variant reported in multiple individuals with Noonan syndrome-like disorder with loose anagen hair 2 (MIM#617506) (ClinVar, DECIPHER, PMID: 33491856). (SP) 1208 - Inheritance information for this variant is not currently available in this individual. (I) Legend: (SP) - Supporting pathogenic, (I) - Information, (SB) - Supporting benign
3billion RCV000490622 SCV002011951 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2023-06-01 criteria provided, single submitter clinical testing The variant is not observed in the gnomAD v2.1.1 dataset. Predicted Consequence/Location: Missense changes are a common disease-causing mechanism. In silico tool predictions suggest damaging effect of the variant on gene or gene product (3Cnet: 0.75). Same nucleotide change resulting in same amino acid change has been previously reported as pathogenic/likely pathogenic with strong evidence (ClinVar ID: VCV000254648 /PMID: 27264673 /3billion dataset). The variant has been previously reported as de novo in at least two similarly affected unrelated individuals (PMID: 27264673, 28211982). The variant has been observed in multiple (>3) similarly affected unrelated individuals (PMID: 27264673, 27681385, 28211982). Therefore, this variant is classified as Pathogenic according to the recommendation of ACMG/AMP guideline.
Laboratory of Medical Genetics, National & Kapodistrian University of Athens RCV000490622 SCV001976798 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2021-10-05 criteria provided, single submitter clinical testing PM2, PP2, PP3, PP5
CeGaT Center for Human Genetics Tuebingen RCV000257986 SCV001962241 pathogenic not provided 2023-04-01 criteria provided, single submitter clinical testing PPP1CB: PS2, PM2, PS4:Moderate, PP2, PP4
Institute of Medical Genetics and Applied Genomics, University Hospital Tübingen RCV000257986 SCV001447547 pathogenic not provided 2020-10-23 criteria provided, single submitter clinical testing
Ambry Genetics RCV001265940 SCV001444112 pathogenic Inborn genetic diseases 2016-07-06 criteria provided, single submitter clinical testing
Equipe Genetique des Anomalies du Developpement, Université de Bourgogne RCV000490622 SCV001190249 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2019-05-04 criteria provided, single submitter clinical testing
Labcorp Genetics (formerly Invitae), Labcorp RCV000257986 SCV000933113 pathogenic not provided 2024-08-15 criteria provided, single submitter clinical testing This sequence change replaces proline, which is neutral and non-polar, with arginine, which is basic and polar, at codon 49 of the PPP1CB protein (p.Pro49Arg). This variant is not present in population databases (gnomAD no frequency). This missense change has been observed in individual(s) with Noonan syndrome-like disorder with loose anagen hair (NSLDH) (PMID: 27264673, 27681385, 27868344, 28211982). In at least one individual the variant was observed to be de novo. ClinVar contains an entry for this variant (Variation ID: 254648). Invitae Evidence Modeling of protein sequence and biophysical properties (such as structural, functional, and spatial information, amino acid conservation, physicochemical variation, residue mobility, and thermodynamic stability) has been performed for this missense variant. However, the output from this modeling did not meet the statistical confidence thresholds required to predict the impact of this variant on PPP1CB protein function. For these reasons, this variant has been classified as Pathogenic.
Blueprint Genetics RCV000257986 SCV000928041 pathogenic not provided 2018-11-07 criteria provided, single submitter clinical testing
Eurofins Ntd Llc (ga) RCV000257986 SCV000861880 pathogenic not provided 2018-06-15 criteria provided, single submitter clinical testing
Center for Pediatric Genomic Medicine, Children's Mercy Hospital and Clinics RCV000257986 SCV000609586 pathogenic not provided 2017-08-30 criteria provided, single submitter clinical testing
GeneDx RCV000257986 SCV000299373 pathogenic not provided 2021-10-05 criteria provided, single submitter clinical testing In silico analysis, which includes protein predictors and evolutionary conservation, supports a deleterious effect; Not observed in large population cohorts (Lek et al., 2016); This variant is associated with the following publications: (PMID: 27264673, 27868344, 27338287, 28211982, 27681385, 28135719, 30368668, 31474318, 31370276, 32476286)
Clinical Genetics DNA and cytogenetics Diagnostics Lab, Erasmus MC, Erasmus Medical Center RCV000257986 SCV001971001 pathogenic not provided no assertion criteria provided clinical testing
Joint Genome Diagnostic Labs from Nijmegen and Maastricht, Radboudumc and MUMC+ RCV000257986 SCV001956324 pathogenic not provided no assertion criteria provided clinical testing
Laboratory of Diagnostic Genome Analysis, Leiden University Medical Center (LUMC) RCV000257986 SCV001798142 pathogenic not provided no assertion criteria provided clinical testing
Diagnostic Laboratory, Department of Genetics, University Medical Center Groningen RCV000257986 SCV001744847 pathogenic not provided no assertion criteria provided clinical testing
Coyote Medical Laboratory (Beijing), Coyote RCV000490622 SCV001441279 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2018-05-28 no assertion criteria provided clinical testing
Lupski Lab, Baylor-Hopkins CMG, Baylor College of Medicine RCV000490622 SCV001438015 likely pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2018-06-29 no assertion criteria provided research
University of Washington Center for Mendelian Genomics, University of Washington RCV001257999 SCV001434812 likely pathogenic Dandy-Walker syndrome no assertion criteria provided research
Centre de Biologie Pathologie Génétique, Centre Hospitalier Universitaire de Lille RCV000490622 SCV001428110 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2019-01-01 no assertion criteria provided clinical testing
Service de Génétique Moléculaire, Hôpital Robert Debré RCV001251211 SCV001426696 pathogenic Noonan syndrome no assertion criteria provided clinical testing
OMIM RCV000490622 SCV000579309 pathogenic Noonan syndrome-like disorder with loose anagen hair 2 2017-05-31 no assertion criteria provided literature only

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