ClinVar Miner

Submissions for variant NM_000546.6(TP53):c.949C>T (p.Gln317Ter)

dbSNP: rs764735889
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Total submissions: 9
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Submitter RCV SCV Clinical significance Condition Last evaluated Review status Method Comment
Molecular Diagnostics Laboratory, Catalan Institute of Oncology RCV000583201 SCV006324629 likely pathogenic Hereditary cancer-predisposing syndrome 2025-08-25 criteria provided, single submitter clinical testing PVS1, PM2_Supporting c.949C>T, located in exon 9 of the TP53 gene, is a nonsense variant expected to result in loss of function by premature protein truncation and nonsense-mediated mRNA decay (PVS1). This variant is located in a mutational hotspot and has been reported in multiple types of tumors (PMID: 33758026, cancerhotspots.org, and internal data). It is not present in the population database gnomAD v2.1.1, non-cancer dataset (PM2_Supporting). The SpliceAI algorithm predicts no significant impact on splicing. According to Giacomelli et al. 2018, it resulted in loss of function with no evidence of a dominant negative effect (PMID: 30224644). To our knowledge, no relevant clinical data have been reported for this variant. This variant has been reported in the ClinVar database (5x pathogenic, 2x likely pathogenic), in the LOVD database (1x pathogenic). Based on the currently available evidence, c.949C>T is classified as a likely pathogenic variant according to ClinGen TP53 Expert Panel Specifications to the ACMG/AMP Variant Interpretation Guidelines for TP53 Version 2.2.0.
Center for Genomic Medicine, Rigshospitalet, Copenhagen University Hospital RCV004669021 SCV005094378 oncogenic Neoplasm 2025-03-04 criteria provided, single submitter clinical testing
Labcorp Genetics (formerly Invitae), Labcorp RCV000615397 SCV002969083 pathogenic Li-Fraumeni syndrome 2024-06-04 criteria provided, single submitter clinical testing This sequence change creates a premature translational stop signal (p.Gln317*) in the TP53 gene. It is expected to result in an absent or disrupted protein product. Loss-of-function variants in TP53 are known to be pathogenic (PMID: 20522432). This variant is not present in population databases (gnomAD no frequency). This variant has not been reported in the literature in individuals affected with TP53-related conditions. ClinVar contains an entry for this variant (Variation ID: 450344). For these reasons, this variant has been classified as Pathogenic.
Ambry Genetics RCV000583201 SCV002686961 pathogenic Hereditary cancer-predisposing syndrome 2025-06-24 criteria provided, single submitter clinical testing The p.Q317* pathogenic mutation (also known as c.949C>T), located in coding exon 8 of the TP53 gene, results from a C to T substitution at nucleotide position 949. This changes the amino acid from a glutamine to a stop codon within coding exon 8. This alteration is expected to result in loss of function by premature protein truncation or nonsense-mediated mRNA decay. As such, this alteration is interpreted as a disease-causing mutation.
Genome-Nilou Lab RCV002289710 SCV002583001 likely pathogenic Li-Fraumeni syndrome 1 2022-06-18 criteria provided, single submitter clinical testing
Genome-Nilou Lab RCV000583201 SCV002582339 likely pathogenic Hereditary cancer-predisposing syndrome 2022-06-18 criteria provided, single submitter clinical testing
Laboratory for Molecular Medicine, Mass General Brigham Personalized Medicine RCV000615397 SCV000731540 pathogenic Li-Fraumeni syndrome 2019-03-13 criteria provided, single submitter clinical testing The p.Gln317X variant in TP53 has not been previously reported in individuals with Li-Fraumeni syndrome or in large population studies. This nonsense variant leads to a premature termination codon at position 317, which is predicted to lead to a truncated or absent protein. Heterozygous loss of function of the TP53 gene is an established disease mechanism in individuals with Li-Fraumeni syndrome. In summary, this variant meets criteria to be classified as pathogenic for Li-Fraumeni syndrome in an autosomal dominant manner based on predicted impact to the protein and absence in controls.
Color Diagnostics, LLC DBA Color Health RCV000583201 SCV000691666 pathogenic Hereditary cancer-predisposing syndrome 2022-03-16 criteria provided, single submitter clinical testing This variant changes 1 nucleotide in exon 9 of the TP53 gene, creating a premature translation stop signal. This variant is expected to result in an absent or non-functional protein product. This variant has been reported in individuals affected with early onset bilateral breast cancer (PMID: 33758026; Color Health internal data). This variant has not been identified in the general population by the Genome Aggregation Database (gnomAD). Loss of TP53 function is a known mechanism of disease (clinicalgenome.org). Based on the available evidence, this variant is classified as Pathogenic.
GeneDx RCV000520579 SCV000618916 pathogenic not provided 2023-03-15 criteria provided, single submitter clinical testing Nonsense variant predicted to result in protein truncation nonsense mediated decay in a gene for which loss of function is a known mechanism of disease; Not observed at significant frequency in large population cohorts (gnomAD); Published functional studies demonstrate evidence for loss of growth suppression ability (Giacomelli et al., 2018); This variant is associated with the following publications: (PMID: 16322298, 18555592, 16000567, 12067251, 23117049, 31447099, 20522432, 35486574, 36269546, 35672466, 34779821, 34188747, 35884448, 36167829, 32817165, 31719099, 30224644, 33758026)

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